Functional characterization of the conserved kinase/ATPase Rio1 in yeast: from kinetochores to its global network and back…

Abstract

The dual-specificity kinase/ATPase Rio1, which is conserved from archaea to humans, is best known for contributing to pre-rRNA processing and the maturation of the small ribosomal subunit. However, in S. cerevisiae we identified Rio1 at kinetochores/centromeres and at the rDNA (nucleolus). I will discuss its roles at both sites and will place these activities within a conserved, global framework that Rio1 manages at the protein, gene, and RNA levels, allowing cells to grow, divide and faithfully transmit its genome in response to stress and nutritional conditions encountered.